This work was supported by la Ligue Nationale contre le Cancer (Equipe labellisée Ligue), ANR-11-LABX-0044_DEEP and ANR-10-IDEX-0001-02 PSL, ANR-12-BSV5-0022-02 “CHAPINHIB”, ANR-14-CE16-0009 “Epicure”, ANR-14-CE10-0013 “CELLECTCHIP”, EU project 678563 “EPOCH28”, ERC-2015-ADG- 694694 “ChromADICT”, ANR-16-CE15-0018 “CHRODYT”, ANR-16-CE12-0024 “CHIFT” and ANR-16-CE11-0028 “REPLICAF”, and “Parisian Alliance of Cancer Research Institutes—PACRI”. Ctf4 is a hub in the eukaryotic replisome that links multiple CIP-Box proteins to the CMG helicase. the H3.3 variant versus the canonical histone H3 by different histone chaperones as well as its mode of incorporation into the chromatin. Martini, E., Roche, D. M., Marheineke, K., Verreault, A. We found that a homotrimerization mutant does not accumulate at local UV damage sites (Fig. Copyright © 2020, American Association for the Advancement of Science. Winn, M. D. et al. Hall, C. et al. & Groth, A. Histone chaperone networks shaping chromatin function. As HIRA has been previously described as a long-lived proteinTo study the impact of HIRA homooligomerization on the interaction with key partners in cells, we generated several HIRA-YFP constructs with single amino acid substitution known to affect the binding of UBN, HIRA(R227K)HIRA homooligomerization is required for CABIN1 interaction. Banumathy, G. et al. Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Se-SAD (single wavelength anomalous diffraction) data of HIRA(644–1017) was collected with an X-ray of wavelength 0.9790 Å at beamline BL41XU (SPring-8, Hyogo, Japan) (Table Initial phases of the HIRA(644–1017) crystal were obtained by the Se-Met single anomalous diffraction (SAD) method using Phenix.autosolCoordinates and structure factors for HIRA(644–1017) have been deposited in the Protein Data Bank under the accession code 5YJE. 1997 Dec;17(12):6953-69. doi: 10.1128/mcb.17.12.6953.Drinnenberg IA, Berger F, Elsässer SJ, Andersen PR, Ausió J, Bickmore WA, Blackwell AR, Erwin DH, Gahan JM, Gaut BS, Harvey ZH, Henikoff S, Kao JY, Kurdistani SK, Lemos B, Levine MT, Luger K, Malik HS, Martín-Durán JM, Peichel CL, Renfree MB, Rutowicz K, Sarkies P, Schmitz RJ, Technau U, Thornton JW, Warnecke T, Wolfe KH.Development. Y.S. RPA interacts with HIRA and regulates H3.3 deposition at gene regulatory elements in mammalian cells. We thank H. Okumura (JASRI) for technical help in the use of beamline BL41XU, T. Ota (KEK) for technical assistance and I. Simeonova for statistical analysis help.Present address: Graduate School of Life Sciences, Tohoku University, 2-1-1, Katahira Aoba-ku, Sendai, Miyagi, 980-8577, JapanThese authors contributed equally: Dominique Ray-Gallet, M. Daniel Ricketts, Yukari Sato.Institut Curie, PSL Research University, CNRS, UMR3664, Equipe Labellisée Ligue contre le Cancer, F-75005, Paris, FranceDominique Ray-Gallet, Ekaterina Boyarchuk & Geneviève AlmouzniSorbonne Université, Institut Curie, CNRS, UMR3664, F-75005, Paris, FranceDominique Ray-Gallet, Ekaterina Boyarchuk & Geneviève AlmouzniDepartment of Biochemistry and Biophysics, Graduate Group in Biochemistry and Molecular Biophysics, Abramson Family Cancer Research Institute, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104, USAStructural Biology Research Center (SBRC), Institute of Materials Structure Science (IMSS), High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki, 305-0801, JapanDepartment of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104, USADepartment of Materials Structure Science, School of High Energy Accelerator Science, The Graduate University for Advanced Studies (Soken-dai), 1–1 Oho, Tsukuba, Ibaraki, 305–0801, JapanYou can also search for this author in
SE data were collected at 4 °C with detection at 280 nm for 2–3 sample concentrations. Dynamics of histone H3 deposition in vivo reveal a nucleosome gap-filling mechanism for H3.3 to maintain chromatin integrity. and Platform for Drug Discovery, Informatics and Structural Life Science, and Basis for Supporting Innovative Drug Discovery and Life Science Research by AMED (Japan) to T.S.
Soni, S., Pchelintsev, N., Adams, P. D. & Bieker, J. J. Amin, A. D., Vishnoi, N. & Prochasson, P. A global requirement for the HIR complex in the assembly of chromatin.
You can also search for this author in Analytical ultracentrifugation experiments were performed with an XL-I analytical ultracentrifuge (Beckman-Coulter) and a TiAn60 rotor with six-channel for sedimentation equilibrium (SE) or two-channel for sedimentation velocity (SV) charcoal-filled epon centerpieces and quartz windows. Tang, Y. et al. Clipboard, Search History, and several other advanced features are temporarily unavailable. and T.S. HighWire
Weiner, M. P. & Costa, G. L. Rapid PCR site-directed mutagenesis.
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